蛋白酶體活性分析試劑盒說明書
產(chǎn)品名稱：蛋白酶體活性分析試劑盒
英文名稱：Proteasome Activity Assay Kit
貨號：K245-100
分類：原裝進口
規(guī)格：100T
英文簡介：Proteasomes are very large (20S, 26S) protein assemblies found in both the nucleus and cytoplasm of all eukaryotes (and in some prokaryotes). They are responsible for the degradation and recycling of proteins which have been previously tagged with ubiquitin. Such tagged proteins are degraded into peptides approximately 7 - 8 amino acids long which are subsequently further degraded. The 20S assembly is the functional protease structure with chymotrypsin-like, trypsin-like and caspase-like protease activities. BioVision’s Proteasome Activity Assay takes advantage of the chymotrypsin-like activity, utilizing an AMC-tagged peptide substrate which releases free, highly fluorescent AMC in the presence of proteolytic activity. The kit also includes a positive control (Jurkat Cell lysate with significant proteasome activity) and a specific proteasome inhibitor MG-132 which suppresses all proteolytic activity due to proteasomes. This permits differentiation of proteasome activity from other protease activity which may be present in samples.
試劑盒中文簡介:蛋白酶體活性分析試劑盒具有檢測類糜蛋白酶活性的優(yōu)勢，利用AMC標記的底物自由釋放的，高熒光強度的AMC來指示蛋白酶的活性.本試劑盒也包含陽性對照（具備顯著蛋白酶體活性的Jurkat細胞裂解液）和一種特異的，可以抑制蛋白酶調(diào)控的所有蛋白水解活性的抑制劑MG-132，這些可以特異的檢測樣本中，可能存在具有蛋白水解作用的蛋白酶體的活性.
產(chǎn)品背景: 蛋白酶體是存在于所有真核生物（部分原核生物）的細胞核與細胞質(zhì)內(nèi)一類巨大蛋白復合物（20s，26s），它的功能是降解或者再循環(huán)被泛素標記的蛋白質(zhì).靶蛋白被降解成為7-8個氨基酸多肽已被隨后進一步降解.20s裝配體帶有類糜蛋白酶，類胰蛋白酶功能蛋白酶結(jié)構(gòu)和類半胱天冬酶活性區(qū)域.
產(chǎn)品特點: 1、簡單的一步法操作，整個實驗僅需30 - 60分鐘；2、準確易用.
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