谷胱甘肽比色檢測試劑盒說明書
產(chǎn)品名稱：谷胱甘肽比色檢測試劑盒
英文名稱：Glutathione Colorimetric Detection Kit
貨號：K261-100
分類：原裝進口
規(guī)格：100T
英文簡介：Glutathione (GSH) is the major intracellular low-molecular-weight thiol that plays a critical role in the cellular defense against oxidative stress in mammalian cells. QIYBIO’s ApoGSHTM Glutathione Colorimetric Assay Kit provides a convenient, colorimetric method for analyzing either total glutathione or the reduced form glutathione alone using a microtiter plate reader. The assay is based on the glutathione recycling system by DTNB and glutathione reductase (Fig. 1). DTNB and glutathione (GSH) react to generate 2-nitro-5-thiobenzoic acid which has yellow color. Therefore, GSH concentration can be determined by measuring absorbance at 412 nm. The generated GSSG can be reduced back to GSH by glutathione reductase, and GSH reacts with DTNB again to produce more 2-nitro-5-thiobenzoic acid. Therefore, the recycling system dramatically improves the sensitivity of total glutathione detection. The kit includes the 5-Sulfosalicylic acid (SSA) for the removal of proteins from samples and for the protection of GSH oxidation and ?-glutamyl transpeptidase reaction. The kit can quantify glutathione from 1-100 ng/well in a increasing reaction time will generate stronger signal. The kit can also specifically detect the reduced form of glutathione (GSH) by omitting the glutathione reductase from the reaction mixture. The sensitivity for detecting the reduced form of glutathione (without recycling system) is 100 times lower than detecting the total glutathione.
試劑盒中文簡介:谷胱甘肽比色度檢測試劑盒提供了一個簡便的測定吸光度的方法，用一個微量滴定讀板儀來分析總谷胱甘肽或者還原型谷胱甘肽的含量.這種分析方法基于由DNTB和谷胱甘肽還原酶組成的谷胱甘肽循環(huán)系統(tǒng).DNTB和GSH反應(yīng)會生成2-N-5-硫苯甲酸，呈現(xiàn)黃色.因此，GSH的濃度可以由測定412nm處的吸光度來確定.生成的GSSG可以由谷胱甘肽還原酶還原成GSH，而GSH與DTNB再次反應(yīng)生成更多的2-N-5-硫苯甲酸.因此，該循環(huán)系統(tǒng)極大地增強了檢測總谷胱甘肽時的靈敏度.試劑盒提供5-磺基水楊酸（SSA），以去除樣品中的蛋白，保護GSH的氧化狀態(tài)，以及γ-谷氨酰胺轉(zhuǎn)肽酶反應(yīng).試劑盒對谷胱甘肽的定量范圍為，一個200I的反應(yīng)中1-100ng/孔.檢測低濃度的谷胱甘肽時，如血液樣品，延長反應(yīng)時間可以產(chǎn)生更強的信號.當(dāng)反應(yīng)體系中不使用谷胱甘肽還原酶后，可以特異的檢測GSH.總谷胱甘肽檢測靈敏度是還原態(tài)谷胱甘肽的檢測靈敏度的100倍.
產(chǎn)品背景: 谷胱甘肽是哺乳動物細(xì)胞中抗氧化脅迫最重要的低分子硫醇.在凋亡早期能觀測到谷胱甘肽的降解.還原型谷胱甘肽（GSH）是人體組織中用于清除反應(yīng)性氧（ROS）的抗氧化劑，氧化型谷胱甘肽（GSSG）在谷胱甘肽還原酶（GR）的作用下可還原成GSH.許多細(xì)胞發(fā)生凋亡時，細(xì)胞漿內(nèi)的GSH水平會降低，此現(xiàn)象發(fā)生在細(xì)胞凋亡非常早的階段，因此檢測谷胱甘肽的水平可以早期判斷和檢測凋亡.
產(chǎn)品特點: 1、步驟簡單，只需要大約2-3小時即可完成檢測；2、操作快速并且方便
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