ADP比色/熒光檢測試劑盒
產品名稱:ADP比色/熒光檢測試劑盒
英文名稱:ADP Colorimetric/Fluorometric Assay Kit
貨號:K355-100
品牌:原裝進口
包裝:100次分析
保存建議: 請在接收到該ADP比色/熒光檢測試劑盒產品后,置于-20℃保存.
產品背景: ADP是ATP脫磷酸后的產物,也能重新加一個磷酸基團轉變成ATP.在ATP與ADP之間的轉化過程中,多種磷酸(酯)酶、磷酸化酶以及激酶均參與其中.ADP在體內通常儲存于血小板中,在釋放后可以與各種嘌呤能受體發(fā)生作用.ADP的水平調控著多種參與中間代謝的酶分子.ADP轉換成ATP通常發(fā)生在線粒體/葉綠體,當然胞漿中也經(jīng)常發(fā)生類似的轉換.傳統(tǒng)的方法是,使用熒光素酶/熒光素介導的方案來檢測ADP的水平.然而,熒光素酶系統(tǒng)不是很穩(wěn)定,并且其檢測設備也不是很普遍,這在一定程度上限制了實驗室里ADP的檢測.
產品描述: 一種ADP比色和熒光分析試劑盒來檢測ADP,操作簡易方便.該試劑盒將樣本內的ADP轉化成ATP和丙酮酸,丙酮酸可以用比色法(λmax = 570 nm)或熒光法(Ex/Em 535/587 nm)定量.該試劑盒簡捷、敏感、穩(wěn)定,并適用于高通量分析,其在生物樣本內的檢測靈敏度可以達到1M ADP
產品特點 1、步驟簡單,只需要1小時不到即可完成檢測;2、操作快速并且方便; 3、該ADP比色和熒光分析試劑盒操作方便、敏感,可適用于高通量分析
ADP is a product of ATP dephosphorylating and it can be rephosphorylated to ATP. Dephosphorylation
and rephosphorylation occur via various phosphatases, phosphorylases
and kinases. ADP is stored in platelets and can be released to interact with a variety of
purinergic receptors. ADP levels regulate several enzymes involved in intermediary
metabolism. ADP conversion to ATP primarily occurs within the mitochondrion and
chloroplast although several such processes occur in the cytoplasm. Conventionally, ADP
levels are measured by luciferase/luciferin mediated assays after ADP is converted to ATP.
However, the luciferase system is unstable and luminescence equipment is not generally
available in most laboratories. BioVision’s newly designed ADP Assay Kit provides a
convenient colorimetric and fluorometric means to measure ADP level. In the assay, ADP
is converted to ATP and pyruvate. The generated pyruvate can be quantified by
colorimetric (λmax = 570 nm) or fluorometric methods (Ex/Em 535/587 nm). The assay is
simple, sensitive, stable and high-throughput adaptable. The assay can detect as low as 1
μM ADP in biological samples.
如與英文說明書有異,以英文說明書為準。
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