異檸檬酸檢測試劑盒說明書
產(chǎn)品名稱：異檸檬酸檢測試劑盒
英文名稱：Isocitrate Assay Kit
貨號(hào)：K655-100
品牌：原裝進(jìn)口
規(guī)格：100T
保存建議: 請?jiān)诮邮盏?/font>異檸檬酸檢測試劑盒產(chǎn)品后，置于-20℃保存.
試劑盒簡介: 異檸檬酸檢測試劑盒可以快速定量檢測各種樣品中異檸檬酸含量，靈敏度高，操作簡單.檢測實(shí)驗(yàn)中，異檸檬酸被氧化，同時(shí)伴有NADPH生產(chǎn)，后者與一種幾乎無色的探針反應(yīng)，產(chǎn)物有色（λmax=450nm）.本試劑盒的檢測量程為1 - 20 nmole.
產(chǎn)品背景: 異檸檬酸（HOOC-CHOH-CH （-COOH）-CH2-COOH）是Krebs TCA循環(huán)中檸檬酸和α-酮戊二酸的中間分子，是植物和一些低等生物中進(jìn)入乙醛酸循環(huán)的分枝點(diǎn).果蔬以及生果蔬食品中異檸檬酸的濃度很.TCA循環(huán)中，異檸檬酸被異檸檬酸脫氫酶氧化形成α-酮戊二酸，同時(shí)生成NAD（P）H.人體缺乏NAD-IDH可能會(huì)導(dǎo)致色素性視網(wǎng)膜炎.
產(chǎn)品特點(diǎn): 1、步驟簡單，只需30分鐘；2、操作快速并且方便；3、該異檸檬酸檢測試劑盒提供了所有所需的試劑以快速、敏感、準(zhǔn)確檢測各種樣本內(nèi)的異檸檬酸含量
Isocitric acid (HOOC-CHOH-CH (-COOH)-CH2-COOH) is an intermediate of the Krebs TCA cycle, positioned between citrate and α-ketoglutarate. It is the branch point from which the glyoxylate shunt operates in plants and lower organisms. Isocitrate is found in substantial concentrations in many fruits and vegetables as well as in foods produced from these raw materials. In the TCA cycle, isocitrate is oxidized by isocitrate dehydrogenase (IDH) to α-ketoglutarate with the generation of NAD(P)H. Loss of NAD-IDH has been implicated as a potential causative factor in retinitis pigmentosa. BioVision's Isocitrate Assay Kit provides a simple, sensitive and rapid means of quantifying isocitrate in a variety of samples. In the assay, isocitrate is oxidized with the generation of NADPH which converts a nearly colorless probe to an intensely colored species with a λmax of 450nm.The Isocitrate Assay Kit can detect 1 to 20 nmoles (~0.2 – 5 μg) of isocitrate.
如與英文說明書有異，以英文說明書為準(zhǔn)。
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