凋亡細(xì)胞分離試劑盒
產(chǎn)品名稱:凋亡細(xì)胞分離試劑盒
英文名稱:Apoptotic Cell Isolation Kit
貨號:K258-30
品牌:原裝進(jìn)口
包裝:30次分析
保存建議: 請?jiān)诮邮盏皆?/font>Biovision品牌的凋亡細(xì)胞分離試劑盒產(chǎn)品后,置于+4℃保存.
產(chǎn)品背景: 細(xì)胞凋亡(apoptosis)的命名主要是根據(jù)某些單個細(xì)胞死亡時細(xì)胞碎裂如花瓣或樹葉散落般的形態(tài)學(xué)特征.目前對細(xì)胞凋亡的認(rèn)識正不斷得到深化,檢測凋亡細(xì)胞的方法也逐漸增多,但形態(tài)改變?nèi)允谴_定細(xì)胞凋亡的最可靠的方法.但是對于凋亡細(xì)胞的分離仍然存在一些困難.
產(chǎn)品描述: 凋亡細(xì)胞分離試劑盒提供了一種簡易有效的方法,通過使用Annexin V/磁珠 (MagBeads),在細(xì)胞培養(yǎng)液或者組織液中分離凋亡細(xì)胞或去除死細(xì)胞.Annexin V是一種鈣離子依賴的磷脂結(jié)合蛋白,對磷酯酰絲氨酸(PS)具有高親和力.PS在凋亡細(xì)胞和死細(xì)胞中會重新從質(zhì)膜內(nèi)部向外部翻轉(zhuǎn).因此Annexin V/磁珠 (MagBeads)中的Annexin V可以通過結(jié)合PS而富集凋亡細(xì)胞,結(jié)合了磁珠的凋亡細(xì)胞由磁力富集,而非凋亡細(xì)胞則仍然處于自由狀態(tài).分離出來的凋亡細(xì)胞和健康細(xì)胞可以用于各種凋亡機(jī)制和通路的研究.本試劑盒也可以用于在細(xì)胞培養(yǎng)時去除死亡細(xì)胞.
產(chǎn)品特點(diǎn): 1、步驟簡單,只需要大約1小時即可完成檢測;2、操作快速并且方便
The Apoptotic Cell Isolation Kit provides a simple and efficient means for isolation of
apoptotic cells or removal of dead cells from cell culture or tissue preparations using annexin
V/magnetic beads (MagBeads). Annexin V is a Ca2+-dependent phospholipid binding
protein with high affinity for phosphatidylserine (PS), which is redistributed from the inner to
the outer plasma membrane leaflet in apoptotic or dead cells. Once on the cell surface, PS
becomes available for binding to annexin V and any of its conjugates. Binding of annexin Vbiotin
to apoptotic cells followed by binding of the biotin to streptavidin-MagBeads enables
separation of apoptotic cells from living cells. The apoptotic cells bound to the MagBeads
adhere to the magnet, while nonapoptotic cells stay in suspension. The separated apoptotic and healthy cells can then be used in a variety of assays to study apoptotic mechanisms and pathways. The kit has also been successfully used to remove dead cells from healthy cell culture.
如與英文說明書有異,以英文說明書為準(zhǔn)。
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